Enrichment of biotinylated peptides. After tryptic digestion, the peptide solution was passed through an immobilized NeutrAvidin column (Pierce) with a bed volume of 50 μl. The column was first ...

Signal comparison with traditional BLI for a peptide When biotinylated TnI aa 23–40 is loaded, followed by anti-TnI at varying doses, the signal with SA XT is 3–5 times higher than with ...

Meanwhile, these antibodies are also valuable research reagents enabling the study of class I peptide ligand presentation and TCR-peptide-MHC interactions. Our biotinylated or unbiotinylated monomers ...