Pellet 106 cells by centrifugation (250 - 300 x g) and carefully remove supernatant Make up 1X of fixation buffer by adding 5 ml of Cytofix (BD554655) to 10 ml of DPBS Add either 300μl (for microwell ...

To identify the cells involved in the process, Chang and his team treated CTLs with supernatants from individually cultured splenocytes. They observed that factors secreted by T cells boosted CTL ...

Indirect ELISAs identify antibodies with the appropriate specificity by immobilizing the antigen on a surface and incubating it with a hybridoma cell supernatant. Researchers also use techniques ...