Background: CRISPR-Cas systems require a protospacer adjacent motif (PAM), which plays an essential role in self/non-self discrimination in their natural context, to ...

Engineered anti-CRISPR proteins for optogenetic control of CRISPR/Cas9 ... in total 6 clusters and the best scoring decoys of the top 3 populated clusters were selected to illustrate the potential ...

So why do we call it CRISPR? Cas proteins are used by bacteria to destroy viral DNA. They add bits of viral DNA to their own genome to guide the Cas proteins, and the odd patterns of these bits of ...

The system consists of a CRISPR array, comprising short direct repeats, separated by short variable DNA sequences (called “spacers”) acquired from foreign genetic elements, and is flanked by various ...

The CRISPR locus is transcribed and processed to generate mature CRISPR RNAs, each encoding a unique spacer sequence. Each crRNA associates with Cas effector proteins that use crRNAs as guides to ...