transform separately pET11a-link-NGFP and pMRBAD-link-CGFP into an E. coli strain that gives high-quality plasmid DNA (for example, DH10B or XL1-Blue) and plate onto selective agar media.

Thus organisms capable of lactose fermentation such as Escherichia coli, form bright pinky-red colonies (plate pictured on the left here). MacConkey agar is commonly used to differentiate between the ...

and lactose broth and agar slants for the completed test. In this procedure, as practiced in our laboratory, we discarded as negative at each step all tubes and plates which we know by previous ...