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如果过松,热盖没有充分接触反应管,就会产生蒸发,影响结果;如果过紧,则会导致反应管变形,更为严重的是可能造成热盖的机械故障,无法使用。而Eppendorf公司的Mastercycler系列的热盖上标有PCR板、0.2ml PCR管,0.5ml PCR管等标识,只要将调档旋钮拧至相应位置 ...
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