Confocal scanning microscopy, developed in the last several decades 2,3, solved this problem by restricting photodetection to light originating from the focal point.

The Pathology Core laboratory is equipped with a Nikon E-1000 upright fluorescent microscope equipped with 4x, 10x, 20x and 40x dry objectives and 40x and 60x oil immersion objectives. The microscope ...

In life sciences, confocal fluorescence microscopy (CFM) is widely regarded for producing high-resolution cellular images. However, it requires fluorescent staining, which poses risks of ...

In trials, POSTECH applied its system to photoacoustic images of human cardiac fibroblasts, and compared the results to corresponding CFM images. The results were "qualitatively and quantitatively ...

While confocal microscopy uses a pinhole to reject out-of-focus light to generate the optical section, a multi-photon (or 2-photon) microscope uses a pulsed infrared laser to stimulate fluorescence ...

This out-of-focus fluorescence is collected by the lens and measured by the detector. As a result, images acquired by wide-field microscopes may appear blurry. Out-of-focus light is the reason why ...

The Leica SP8 FALCON (FAst Lifetime CONtrast) is a fast and completely integrated fluorescence lifetime imaging microscopy (FLIM) confocal platform. SP8 FALCON delivers video-rate FLIM with pixel ...

The basic scheme here is known as confocal laser scanning fluorescence microscopy, where a laser at one wavelength excites fluorescent tags bound to structures in a sample. Light emitted by the ...

Fluorescent microscopy is a robust and versatile technique offering enhanced selectivity over standard confocal approaches.

This confocal microscope is ideal for short- and long-term imaging of live cells and tissues ... The LSM700 supports state-of-the-art imaging applications including colocalization studies, volume ...

The MEMS confocal unit can be connected to an inverted microscope to allow for confocal fluorescence imaging. Depending on the needs, the MAICO line-up provides three variations with varying ...

The Nikon A1R-HD25 Confocal has Four channel imaging, high speed resonance scanner (30 fps at 512x512, up to 420 fps depending on area), perfect focus system for timelapse imaging, live cell imaging ...