The Pathology Core laboratory is equipped with a Nikon E-1000 upright fluorescent microscope equipped with 4x, 10x, 20x and 40x dry objectives and 40x and 60x oil immersion objectives. The microscope ...

Confocal scanning microscopy, developed in the last several decades 2,3, solved this problem by restricting photodetection to light originating from the focal point.

The basic scheme here is known as confocal laser scanning fluorescence microscopy, where a laser at one wavelength excites fluorescent tags bound to structures in a sample. Light emitted by the ...

This out-of-focus fluorescence is collected by the lens and measured by the detector. As a result, images acquired by wide-field microscopes may appear blurry. Out-of-focus light is the reason why ...

While confocal microscopy uses a pinhole to reject out-of-focus light to generate the optical section, a multi-photon (or 2-photon) microscope uses a pulsed infrared laser to stimulate fluorescence ...

A confocal microscope is a kind of fluorescent microscope used to obtain high-quality 2D and 3D images. The device is most commonly used to view cells and cell structures, but can be used to ...

Current concepts in light and electron microscopy and scanning probe techniques. Theory and practice of fluorescence (including confocal and multi-photon), atomic force, scanning and transmission ...

Fluorescent microscopy is a robust and versatile technique offering enhanced selectivity over standard confocal approaches.

The MEMS confocal unit can be connected to an inverted microscope to allow for confocal fluorescence imaging. Depending on the needs, the MAICO line-up provides three variations with varying ...

Their findings are published in Nature Communications. In life sciences, confocal fluorescence microscopy (CFM) is widely regarded for producing high-resolution cellular images. However ...

spinning disc and laser scanning confocal microscopes, 2-photon systems, and super-resolution fluorescence imaging systems. The facility also includes an analysis suite with computers that have ...