To site-specifically label proteins in living cells, researchers mutate an enzyme to recognize a small fluorophore and catalyze its attachment to a small peptide tag. Fluorescent protein fusions ...

This obstacle has now been cleared by the Stefan Kubicek-led research team at CeMM: Five different fluorescent colors can be used to label multiple proteins at once using a technique called ...

Using UbiREAD, scientists label fluorescent proteins with specific ubiquitin codes and track their degradation in cells. The study, published in Molecular Cell, revealed which ubiquitin code can ...

Lastly, the team wanted to compare their method to transgenic labeling, a technique commonly used to view single cells in intact tissues. However, the fluorescent marker used in this technique can be ...

Keren and her team decided to rely on what is known as a combinatorial approach: labeling each protein by attaching to it several fluorescent tags, so that each molecule is identified by a unique ...

For patients with polycystic kidney disease (PKD), a common genetic disorder that ravages the waste-removing organ with cysts, dialysis and transplantation are among the only treatments. More than 12.

By deploying a green fluorescent protein that binds to polycystin-2 ... The tracking method Nikonorova used, known as "proximity labeling," helped her determine the precise mechanism by which ...

For patients with polycystic kidney disease (PKD), a common genetic disorder that ravages the waste-removing organ with cysts, dialysis and transplantation are among the only treatments.