To maintain ploidy, unidirectional progression through the phases of the cell cycle (G1 → S → G2 → M → G1) is essential. To keep cells from regressing, the transitions of the mitotic cell ...

This sequential expression followed the phases of the traditional cell cycle: G0/G1 (the cell grows larger), S (the cell replicates its DNA), and G2/M (the cell prepares for division and then splits).

The stain can be applied to live, detergent-permeabilized or fixed cells for quantification of their distribution among the three main phases of the cell cycle (G 0 /G 1, S and G 2 /M).

HIV-1 Vif induces a unique and prolonged pseudo-metaphase arrest by disrupting phosphatase feedback regulation at the kinetochore.