METHODS Fibronectin, elastin, and glial fibrillary acidic protein (GFAP) staining were assessed in young and old laminae cribrosae. An age range (7 days to 96 years) of human laminae cribrosae were ...
Similar alteration of the GFAP staining was found in the hyperglycaemic (Stz+/Ins-) and insulin-treated hyperglycaemic (Stz+/Inz+) groups. Intravitreal PACAP resulted in suppression of the elevated ...
For GFAP staining, cells were washed 3 times with PBS, followed by PBS with 0.5% Triton X-100 and PBS with 0.5% Triton X-100 and 2% heat-inactivated goat serum. Cells were incubated with ...