A method to select for proteins that are highly efficient at folding yields 'superfolder' GFP, an optimized GFP for use in protein fusions. Fusion proteins are powerful tools for the elucidation ...
supercharged GFP! Supercharged GFP may not be faster than ... David Liu's group at Harvard University discovered that mutants containing large numbers of charged, surface-exposed residues were ...
“That indicated there must be some kind of coupling,” Morin said. Shimomura continued to study GFP and identified its chromophore in 1979. 4 Ultimately, though, he returned to studying bioluminescence ...
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New Class of CRISPR-Cas Enzymes for Precise Genome EditingThe research team then created nine single amino acid mutant variants of ChCas12b by ... to hydrogen bonding with target DNA-sgRNA. The GFP reporting system was then used to test the specificity ...
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