If Chalfie and Euskirchen wanted only the GFP DNA, they could amplify just the GFP gene by PCR, but that risked introducing mutations since PCR was error-prone at the time. Alternatively, they could ...

1) Figure 1: GFP-fragment reassembly vectors and cloning ... which we prefer for libraries, we perform colony PCR with primers 5′-tagcggatcctacctgacgc-3′ and 5′-ttcgggctttgttagcagcc-3′.

The discovery of the green fluorescent protein (GFP), increasingly sophisticated ... The polymerase chain reaction (PCR) technology, for example — the simple elegance of which made many of ...

21%), the KI efficiency was found to be 80% higher. GFP KI at RAB11A Locus in HEK293 cells using electroporation with 5 ug of PCR dsDNA and GenWand™ dsDNA templates. Image Credit: GenScript ...