Avoid fusions to the GFP fragments at the site of protein-protein interaction and consider optimizing the spacer length between the fusions and GFP fragments, if necessary. In the absence of ...

The data convincingly show that the transcription factor Sp1 regulates ephrin-mediated axon guidance in the spinal cord. Although the authors show that Sp1 and its co-activators p300 and CBP are ...

The recent findings of Waldo et al. 1 demonstrate that the fusion of the green fluorescent protein (GFP) to insoluble proteins dramatically reduces its folding ability in prokaryotic cells.