The recent findings of Waldo et al. 1 demonstrate that the fusion of the green fluorescent protein (GFP) to insoluble proteins dramatically reduces its folding ability in prokaryotic cells.

1) Figure 1: GFP-fragment reassembly vectors and cloning ... standing column with 2 ml of wash buffer and then elute His-tagged protein with wash buffer plus 300 mM imidazole.

Baird informed Tsien of his mistake. “He basically had all of the ideas for what you would do to exploit a serendipitous finding,” Baird said. Ultimately, the team found that breaking GFP and ...