Determine the protein concentration of samples using the bicinchoninic acid procedure 6 ... Store the fractions at 4 °C for subsequent RP-HPLC or SDS-PAGE or at −20 °C for long-term storage.

The contents of each flask were then diluted to volume with HPLC-grade water. A 5-mL portion of each diluted sample was further diluted to 50 mL with HPLC-grade water and 20 µg of internal ...