One of the central challenges of rAAV production is the need for a ‘helper virus’ to facilitate replication within a host mammalian cell. In the early days of rAAV, cultured cells would first ...

The same is true of hepatitis D virus. And like other virusoids, it is also understood to be dependent on the envelope proteins of a helper virus —in this case hepatitis B virus— for ...

In this manufacturing process, the baculoviruses play a dual role, functioning as the ‘helper’ virus normally required for replication, as well as the vehicle for AAV genetic material.