1| Pellet ∼10 8 cells at 300g at 4 °C for 10 min in a centrifuge. The nuclei should be kept cold throughout the procedure. Manipulations and transport to the centrifuge should be done on ice.

The ARCUS gene-editing platform is derived from the nuclease I-CreI of the green alga Chlamydomonas reinhardtii. This nuclease evolved naturally to perform a single, highly specific DNA edit.