Always check your PCR fragments on agarose gel. If you have any secondary bands - however faint - the fragment must be purified from gel. Large templates (cosmids, BACs, PACs) We require 500 ng – 1µg ...

DNA sequencing is a laboratory method used to determine the sequence of a DNA molecule. The method was developed by Frederick Sanger in ... their sizes. The bands of the gel are detected, and ...

The polymerase chain reaction (PCR) and sequencing are intricately linked and vital to many fields in the life sciences. First invented in 1983, PCR is used to dramatically amplify DNA in a matter of ...

FIMM Technology Centre's SeqLab provides DNA sequencing services for the scientific community nationally and internationally. In addition to DNA sequencing, small to medium scale fragment analysis ...

producing a single PCR product that is less than 1000 bases, while the 2.3 to 2.5 kilobase PD2 segment needs to be split into several smaller amplicons to enable Sanger sequencing.Sanger ...