A restriction map of the 5.3 kb insert is prepared. The recombinant plasmid is digested with the enzymes listed above in single digests, and then several combinations of enzymes are tested in double ...

A scaleable method for the liquid-phase separation of plasmid DNA from RNA ... and was well digested by restriction enzymes EcoRI and HindIII under standard conditions (data not shown).

It should hybridize to the template DNA 250–500 nucleotides 3′ of the position to be cleaved by the chosen restriction enzyme ... strand of a recombinant plasmid containing the DNA sequences ...