Fluorescence microscopy has undergone a renaissance in the last decade. The introduction of green fluorescent protein (GFP) and two-photon microscopy has allowed systematic imaging studies of ...

In concert with microscopy this permitted previously ... There is thus a greater need for users to understand the principles of fluorescence and how different imaging systems work so biologists ...

In modern life science research, fluorescence microscopy is a popular and powerful imaging technique. Yet, despite its overall success, the need for fluorescent labels limit its potential applications ...

In STED microscopy, the sample is labeled with fluorescent dyes or probes that can be excited by a specific wavelength of light. When excited, these fluorophores emit light at a longer wavelength, ...