The crux of the method involves co-expression of the control (dsRed) and target (enhanced green fluorescent protein (EGFP)) fluorescent reporters separated by an internal ribosomal entry site on a ...

Now, a just-developed enhancement is making it even better by improving yields for myriad recombinant proteins, including enhanced green fluorescent protein (eGFP) and human cytochrome c.

In addition, the inventors inserted an eGFP gene into the first gene position (promoter proximal). The resulting virus is replication-competent and efficiently expresses GFP in infected cells.