Our laboratory is dedicated to understanding the consequences of activation of these pathways and to using this information to develop mechanism-based therapeutic strategies. The laboratory is ...

Cell apoptosis and cell cycle were analyzed by flow cytometry, and the levels of genes and proteins of the Raf/MEK/ERK pathway were determined by RT-PCR and Western blotting.

The review explores strategies to overcome this resistance, focusing on targeting downstream molecules within the RAS-RAF-MEK-ERK and PI3K-AKT-mTOR pathways. Furthermore, the study explores the ...

a RAF/MEK clamp, with a pan-RAF inhibitor led to strong tumor regressions in multiple NRAS- and BRAF-driven tumor models corresponding with nearly complete shutdown of RAS/MAPK pathway signaling ...