In addition, SYBR Green I cannot be used in multiplexed ... An advance in the technology has been the addition of a DNA minor groove–binding (MGB) moiety at the 3′ end of the TaqMan probes ...

We have developed a two-step, real-time, quantitative assay, using the polymerase chain reaction with reverse transcription (RT-PCR) and based on SYBR Green I monitoring of PCR product ...