Hieff® qPCR SYBR Green Master Mix(High Rox Plus)是2×实时定量PCR扩增的预混合溶液。Mix中含有热启动Hieff® DNA Polymerase、SYBR Green I、dNTPs、Mg2+及High Rox。使用时,仅需在扩增体系中加入模板和引物即可进行实时荧光定量PCR,大大简化操作过程,降低污染几率。 本品采用的DNA ...
We have developed a two-step, real-time, quantitative assay, using the polymerase chain reaction with reverse transcription (RT-PCR) and based on SYBR Green I monitoring of PCR product ...
Real-time PCR (qPCR) is the gold-standard for sensitive, specific detection and quantification of nucleic acid targets through fluorescence-based techniques. TaqMan assays and SYBR Green dye are two ...