A Sanger sequencing reaction is run with a single primer. The following table lists the recommended amount of DNA template and primer for optimal Sanger sequencing results. Note: for plasmid DNA the ...

Due to the use of dideoxynucleotides in the reactions, Sanger sequencing is also called "dideoxy" sequencing.

When Sanger sequencing was first introduced, four separate reagents were used, one for each type of ddNTP. The four reaction products were then separated by gel electrophoresis, a process that ...