The breakthrough came from the work of Camillo Golgi (Timeline) who, in 1873, developed a silver nitrate staining method — the 'reazione nera' — that made it possible to see all of the ...

In slides stained with Dieterle silver nitrate stain and/or anti-Bb antibodies, sectioned filaments embedded in epithelial cells were observed in samples of all callus material and in the biopsy ...