From that point forward, resolution proceeds as in the single-stranded model (Figure 3). (Note that a third model of recombination, synthesis-dependent strand annealing [SDSA], has also been ...
If two homologous regions flank the DSB, they can anneal after being exposed by DNA resection (single-strand annealing; SSA), which causes the deletion of the intervening region. An additional ...
The temperature is lowered to around 50-65°C, enabling the primers to anneal specifically to their complementary sequences on the single-stranded DNA template. The optimal annealing temperature ...
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