Researchers show that superpositively charged GFP enters mammalian cells with ease and can be used as a nucleic acid delivery vehicle. It's a bird, it's a plane, it's ... supercharged GFP!
Gene therapy in the central nervous system (CNS) is hindered by the presence of the blood–brain barrier, which restricts access of serum constituents and peripheral cells to the brain parenchyma.
This requires the ability to accurately track cells, observing their function, movement and interactions in vitro and in vivo. GFP ioMicroglia is built from the company’s well-established ioMicroglia.
“We didn’t do it with an idea of doing big technological breakthroughs or screens ... Through this spurious connection, ClonTech optimized GFP for expression in mammalian cells, licensed, and ...