Peripheral T-cell ... protein (GFP) Lentiviral vector, using Lipofectamine 2000 (Life Technologies, Carlsbad, CA, USA) per the manufacturer’s protocol. Prior to lentiviral transduction ...
A lot of genes deregulated in malignant plasma cells (PCs) involved in multiple ... malignant PCs and to genetically modify them. Usual lentiviral vectors (LVs) pseudotyped with vesicular ...
Viral gene delivery is a ubiquitous laboratory technique with a myraid of uses. Understanding the basics of gene delivery workflows, from vector selection and cloning ...