In one of these, namely, double embedding in nitrocellulose and paraffin, the tissue is first infiltrated with an ether–alcohol solution of nitrocellulose ; the volume occupied by the solvent is ...

involves tissue fixation, embedding and sectioning. Cells are typically fixed with 2% glutaraldehyde (e.g. in 100 mM Na-cacodylate or Na-phosphate buffer, pH 7.4) for 1-2 hours at RT post-fixed with 1 ...

In general, biopsy pads should not be used with larger tissue samples. However, they can be used to maintain orientation or positioning of trimmed samples, as distortion is of less concern with fixed ...

Frozen tissues can be stored in a -80 freezer. If the tissues were frozen in a flammable freezing medium, take care to allow it to evaporate before placing the blocks in the freezer. Even if frozen in ...