The most recent and sophisticated multi-component transfection reagent, FuGENE ® 4K, was created to transfer DNA into both difficult and routine mammalian cell lines. Users are able to obtain ...

Download this application note from Thermo Fisher Scientific to discover an approach using the Neon NxT system that researchers can follow for optimal transfection results.

1| Harvest exponentially growing cells 24 h before transfection by trypsinization and replate them at a density of 1 × 10 5 to 4 × 10 5 cells/cm 2 in 60-mm tissue culture dishes or in 12-well ...

Furthermore, the transfection procedures used to deliver the three plasmids to cells are robust, well-established, and can be performed at manufacturing scale. Today, at least 11 companies are ...