Then, in the late-1980s, a new option emerged: the yeast two-hybrid (Y2H) assay. The brainchild of Stanley Fields, a Howard Hughes Medical Institute investigator at the University of Washington, Y2H ...

But yeast two-hybrid assays and coimmunoprecipitation, the two main techniques for generating "interactomes," maps of protein interactions on a proteome-wide scale, can leave gaping holes. The yeast ...