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联合治疗新突破:异体自然杀伤细胞与西妥昔单抗协同抗击头颈部 ...
在这项研究中,研究人员主要运用了以下几种关键技术方法:一是细胞培养与扩增技术,从健康供体的外周血单个核细胞中分离出 CD3-CD56 + 细胞,经过多步培养和扩增,获得用于实验的异体 NK 细胞;二是细胞毒性检测技术,采用 calcein - AM 染色法,检测 NK 细胞对 ...
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