For example, for the type I-F CRISPR/Cas system, Cas1 and Cas2 proteins first insert a short segment 'spacer' derived from the foreign DNAs into the CRISPR locus. Subsequently, the CRISPR array is ...

So why do we call it CRISPR? Cas proteins are used by bacteria to destroy viral DNA. They add bits of viral DNA to their own genome to guide the Cas proteins, and the odd patterns of these bits of ...

This gene-editing tool has been adapted for molecular diagnostics, utilizing Cas proteins to detect specific nucleic acid sequences with high sensitivity. CRISPR-based platforms such as SHERLOCK ...

View Full Profile. Learn about our Editorial Policies. Since the bacterial-origin CRISPR-Cas system rose to popularity as a genome editing tool, scientists have wondered whether other genome editors ...

The CRISPR locus is transcribed and processed to generate mature CRISPR RNAs, each encoding a unique spacer sequence. Each crRNA associates with Cas effector proteins that use crRNAs as guides to ...