A method to select for proteins that are highly efficient at folding yields 'superfolder' GFP, an optimized GFP for use in protein fusions. Fusion proteins are powerful tools for the elucidation ...

The recent findings of Waldo et al. 1 demonstrate that the fusion of the green fluorescent protein (GFP) to insoluble proteins dramatically reduces its folding ability in prokaryotic cells.

The model was able to predict optimized GFP sequences that had as many as seven different amino acids from the protein ...