F. Lysates from U2OS cells stably expressing EGFP (control) and U2OS dKO cells expressing SYTL5-EGFP and/or mScarlet-RAB27A (wild-type, T23N or Q78L mutants) were immunoprecipitated using GFP-Trap ...

A method to select for proteins that are highly efficient at folding yields 'superfolder' GFP, an optimized GFP for use in protein fusions. Fusion proteins are powerful tools for the elucidation ...

A temporally resolved CRISPR-Cas9 screen reveals DAC-induced DNA damage drives trans-cell cycle cytotoxicity that depends on ...

Louis, MO, USA), and fluorescence was determined every 10 sec using a TECAN SPARK microplate reader. LAD2 cells were transfected with human LysRS (KARS WT) pcDNA 3.1+C-eGFP vector and GFP empty vector ...

In this valuable paper, the authors created a reporter mouse line in which the Axon Initial Segment (AIS) is intrinsically labeled by an ankyrin-G-GFP fusion protein activated by Cre recombinase, ...

Myeloid deficiency of the mitochondrial transacetylase tafazzin upregulates mtROS, disrupting UPR and calcium homeostasis, ...

PRV-805NEW伪狂犬病毒（PRV）广泛应用于示踪跨多个突触的神经环路。通过将PRV注射到外周部位，如心脏、胃肠道和肝脏，可以追踪到调控这些部位的大脑核团。此外，PRV也可以直接注射在中枢神经系统，用来示踪多突触神经环路。然而，由于病毒感染多个不 ...

Tata Institute of Fundamental Research, Homi Bhabha Road, Colaba, Mumbai 400005, India ...

为解决无法预测单细胞特性的问题，美国加利福尼亚大学圣地亚哥分校的研究人员开展用 AI 分析 3D 成像流式细胞仪数据预测细胞特性的研究。结果显示预测高蛋白质表达细胞准确率达 88% 等。该研究对医学和生物研究意义重大，值得一读。