This was peformed using an in-house immunoaffinity chromatography column embedded with the anti-clamp monoclonal antibody (mAb) HIV1281 coupled to 1 or 5 ml HiTrap-NHS activated HP columns (Cytiva).
Three early-career researchers thrown together on Sable Island, a treeless island in the North Atlantic, think others can learn from their experiences.
Phosphorylation is the most abundant post-translational modification, regulating several aspects of protein and cell function. Quantitative phosphoproteomics approaches have expanded the scope of ...
(22,23) The peptides were reconstituted in 100 μL of high-pH buffer A (5 mM ammonium hydroxide in water, pH 10) and were separated on an XBridge Peptide BEH C18 column (Waters) connected to an Accela ...