After purification using the QIAquick PCR Purification Kit (QIAGEN, United States), both products from the odd and even mixtures were pooled for sequencing (Figure 1). The second-strand cDNA was ...

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Stoddart Institute of Molecular Science, Department of Chemistry, Zhejiang University, Hangzhou 310058, P. R. China Zhejiang-Israel Joint Laboratory of Self-Assembling Functional Materials, ...

PCR-based techniques are methods that rely on the polymerase chain reaction (PCR) to amplify stretches of DNA by creating many identical or near-identical copies. For example, PCR amplification ...

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Third, every 24 RILs were pooled together and randomly sheared ultrasonically [Covaris S2 (Covaris, Inc.)], and QIAquick PCR Purification kit was applied for purifying sheared DNA fragments. The ...