The coated plates were blocked with 5% skim milk in phosphate-buffered saline with 0.05% Tween 20 (PBST) for 1 h at 37°C, washed with PBST three times, then incubated with 100 μL of serum samples ...

The histone lysine methyltransferase DOT1L (DOT1-like histone lysine methyltransferase) is responsible for the epigenetic regulation of gene expression through specific methylation of lysine79 residue ...

At times when there were disagreements in diagnosis, WE discussed the case with the data collectors and the consensual diagnosis was taken. An electronic template was prepared using Epidata V.3.01 ...