Plasmid DNA (pDNA) is a key material for producing gene therapies, mRNA and DNA vaccines. The alkaline lysis process to isolate pDNA is simple on the benchtop, but it’s challenging at industrial scale ...

Inside cells, DNA is under/overtwisted, resulting in the formation of 'supercoiled' loops, resembling what can happen to long cords or garden hoses in our homes." In contrast with relaxed DNA ...

Upon infection, cccDNA is generated as a plasmid-like episome in the host cell nucleus from the protein-linked relaxed circular (RC) DNA genome in incoming virions. Its fundamental role is that as ...

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The targeting mechanism facilitated the interaction of NPs with target cells, thereby enhancing their internalization and subsequent exogenous plasmid DNA (pDNA) translation and protein expression. In ...

2 Department of Developmental Biology and Cancer Research, The Institute for Medical Research Israel-Canada, The Hebrew University-Hadassah Medical School, Jerusalem, Israel Objective Growing evidence ...