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普通 taq 酶和高保真酶有何差异
普通 taq 酶和高保真酶在保真度、扩增效率、延伸能力、错配率以及价格等方面存在区别。 1. 保真度:高保真酶具有更高的保真度,能够更准确地复制 DNA 模板,减少碱基错配的发生。普通 taq 酶的保真度相对较低。 2. 扩增效率:普通 taq 酶的扩增效率通常较高,能快速扩增 DNA 片段。而高保真酶的扩增效率可能略逊一筹。 3. 延伸能力:普通 taq ...
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