and 0.5 U FastStart PCR Taq DNA Polymerase (Roche). The thermal profile was as follows: 10 min at 95°C, then 40 cycles of 20 s at 95°C, 30 s at 60°C, 40 s at 72°C and a final step of 5 min at 72°C.

The first PCR was performed on a volume of 25 µL containing: 1× reaction buffer, 1.5 mM MgCl 2, 200 µM dNTPs, 10 pmol of each primer (Mbcr1: 5′-GAAGTGTTTCAGAAGCTTCTC-3′; 2oabl1: 5′-TGATTAAGCCTAAGACCC ...

5’ATTACCGCGGCTGCTGG3’), and 5 U of Hot Start TaKaRa Taq Polymerase. The forward primer contains 40 base pair (bp) of high G+C content (a GC clamp), which prevents complete dissociation of the DNA ...

Expert Rev Mol Diagn. 2012;12(7):731-754.

Binding buffer: 5 mM Tris-HCl (pH 7.5), 0.5 mM EDTA, 1 M NaCl Breaking buffer: 10 mM Tris-HCl (pH 7.5), 1% Triton-X 100, 1% SDS, 100 mM NaCl, 1 mM EDTA ...

As the ISC 2025 exams approach, students are looking for expert advice on how to score high marks in the three core Science ...

In recent years, quantitative real-time PCR tests have been extensively developed in clinical microbiology laboratories for routine diagnosis of infectious diseases, particularly bacterial diseases.

PCR was performed by using bisulfite-treated DNA as templates. The sequences of interest were amplified using polymerase Takara Ex Taq Hot Start (Clontech, Mountain View, CA) and methylation-neutral ...

This week, host Wendy Corr has a conversation with Dr. Hudson Freeze, whose scientific studies in the 1960s at Yellowstone ...

3). In PGP gene expression profile studies, good quality RNA was isolated from all the four actinomycete strains. qRT-PCR analysis on selected PGP genes of actinomycetes revealed up-regulation of all ...