CRISPR-Cas9 genome editing exploits the CRISPR-Cas system to modify a genome in a targeted manner. Guided by RNA, the Cas9 endonuclease breaks DNA at a target sequence. Imprecise repair of the ...

Gene-editing strategies involving engineered nucleases [i.e., zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), meganucleases, and clustered regularly interspaced ...

CRISPR-Cas systems are prokaryotic immune systems ... when delivered with adeno-associated viral gene therapy. SeqCas9, a Cas9 variant from the Streptococcus family, recognizes a simple NNG ...