Viral gene delivery is a ubiquitous laboratory technique with a myraid of uses. Understanding the basics of gene delivery workflows, from vector selection and cloning to transduction, is a fundamental ...

Usual lentiviral vectors (LVs) pseudotyped with vesicular stomatitis virus envelope glycoprotein poorly transduced healthy and malignant PCs. Here, we report that LVs pseudotyped with the ...

No. GMP-CM3101) exhibited a lentiviral transduction efficiency comparable to that of the other medium. Human PBMCs were cultured for two weeks in either CelThera TM GMP T Cell Expansion Medium ...

To improve the efficacy of gene therapy when using anti-sickling beta globin gene transfer, they added cyclosporin (CsH) to increase transduction by inhibiting lentiviral restriction factors.

A new vector system known as lentiviral vectors can permanently integrate the gene into dividing and non-dividing cells. The capacity of lentiviral vectors to reliably integrate at various genomic ...

Lentiviruses have the unique ability amongst retroviruses of being able to infect non-cycling cells. Vectors derived from lentiviruses have provided a huge advancement in technology and seemingly ...

L. Naldini, U. Blomer, P. Gallay, D. Ory, R. Mulligan, F.H. Gage, I.M. Verma, D. Trono, "In Vivo Gene Delivery and Stable Transduction of Nondividing Cells by a ...

Here we asked if increasing NaCl tonicity in the vector formulation enhances the transduction efficiency of 3 gene therapy vectors: adenovirus, AAV, and lentiviral vectors. We observed that vectors ...