Adjust the reaction volume to 500 μl with water and purify the ligated genomic DNA using a QIAquick PCR purification kit. Store the purified DNA at a concentration of 1 μg/μl. To elute the ...
Cut out the gel slice that includes bands greater than 1 kb for each sample and purify the DNA using QIAquick Gel Extraction ... and then transfer the PCR tube to ice. The samples may be stored ...
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