“Products created during setup decrease the efficiency of PCR,” says Joseph Donnenhoffer of Roche. The company's FastStart Taq polymerase has a one-base deletion that renders the enzyme ...

There are essentially two amplification approaches: one uses PCR with a polymerase such as Taq and the other is multiple displacement amplification using the Phi29 polymerase. PCR-based methods ...

She is passionate about making complex scientific concepts accessible to readers. View Full Profile. Learn about our Editorial Policies. Polymerase chain reaction (PCR) is a fundamental molecular ...

Before PCR, the process of DNA sequencing was very lengthy and quite expensive. How did the discovery of the Taq polymerase result in this important research technique? To amplify a DNA fragment ...

Today, PCR is ubiquitous in molecular biology laboratories ... Cetus Corporation scientists David Gelfand and Susanne Stoffel purified a thermostable DNA polymerase from Thermus aquaticus—the ...