引言在哺乳动物的胚胎发育过程中，内胚层（endoderm）是形成重要器官的基础，特别是呼吸系统和消化系统。然而，内胚层如何从一个简单的细胞层分化为这些复杂器官的过程，直到今天依然充满了未知。随着研究手段的进步，研究人员逐渐揭开了这一复杂过程的面纱。1 ...

Traditional bulk RNA sequencing and microarray technologies can only provide average expression levels ... Principal component analysis (PCA) was then performed for dimensionality reduction, followed ...

RNA分子作为生命活动的重要组成部分，其结构的多样性和动态性对功能的实现至关重要。然而，RNA分子的高异质性和柔性使得解析其三维结构成为 ...

Single-cell RNA sequencing (scRNA-seq) has become one of the most widely used technologies in basic biology. With the rise of scRNA-seq, the use of UMAP has become ubiquitous in publications. While ...

NIS-Seq的核心原理围绕在细胞核内生成明亮且稳定的信号。传统的原位测序依赖于细胞胞质中的信使RNA（mRNA），然而mRNA的表达水平高度依赖细胞的 ...

“The more we look, the crazier we see,” said Mark Peifer, a cell and developmental biologist at the University of North Carolina. They resemble what scientists call viroids, which are infectious loops ...

This work introduces an important new method for depleting ribosomal RNA from bacterial single-cell RNA sequencing libraries, demonstrating its applicability for studying heterogeneity in microbial ...

Thus, we address a persistent challenge in bacterial single-cell RNA-seq regarding rRNA abundance, exemplifying the utility of this method ... Expression levels of different marker genes across ...