The virus-antibody mixtures were transferred to the 96-well plates containing the cell monolayers. The luciferase assay was performed after incubation for 48 h at 37°C, four replicate wells per ...

Bioluminescence, the natural emission of light by living organisms, is powered by a chemical reaction catalyzed by an enzyme, typically a luciferase, acting on a bioluminescent substrate.

Herein we describe a DNA biosensor that provides a sensitive readout for such low-copy DNA sequences through proximity-mediated reassembly of two independently optimized fragments of NanoLuc ...