In patient-derived and animal tumour models, mubritinib suppressed glioblastoma stem cells and tumour growth by targeting ...

RNA was isolated using TRIzol reagent, and cDNA synthesis was conducted using the PrimeScript™ RT kit. Gene expression was analyzed with SYBR qPCR Master Mix on a Roche LightCycler 480 system. GAPDH ...

Real-time PCR (qPCR) is the gold-standard for sensitive, specific detection and quantification of nucleic acid targets through fluorescence-based techniques. TaqMan assays and SYBR Green dye are two ...

The conserved sequences of GI and GII subtypes were retrieved to design the primers and probe. Leveraging this information, we developed a TaqMan probe-based quantitative real-time PCR (qPCR) assay ...

Excalidraw is an innovative online whiteboarding tool that uses the power of artificial intelligence (AI) to convert simple text prompts into detailed, professional-quality diagrams. Whether you ...

Quantitative RT-PCR was performed in triplicates for each sample, and SYBR Green I Master Kit (Roche; Cat #04887352001) was used to carry out the reaction. qRT-PCR was performed using the LightCycler ...