mCherry is a member of the mFruits family of monomeric red fluorescent proteins (mRFPs). As an RFP, mCherry was derived from DsRed of Discosoma sea anemones, unlike green fluorescent proteins (GFPs) which are often derived from Aequorea victoria jellyfish. [1]

2022年8月18日 · mCherry is a basic (constitutively fluorescent) red fluorescent protein published in 2004, derived from Discosoma sp.. It is reported to be a very rapidly-maturing monomer with low acid sensitivity.

The short mCherry isoform creates in average a 19% background fluorescence compared with the original sequence. The control shows the background fluorescence of the S. cerevisiae uracil-deficient strain. Each measurement was performed in biological triplicates.

What distinguishes mCherry from other proteins is its optimal balance of photostability, brightness, and excellent pH resistance, making it the most widely used red fluorescent protein in biomedical research. mCherry fluorescence is a constitutive and inherent characteristic of this protein.

MCherry, the Discosoma sp. mushroom coral-derived monomeric red fluorescent protein (RFP), is a commonly used genetically encoded fluorophore for live cell fluorescence imaging.

The red fluorescent protein mCherry is of considerable interest for fluorescence fluctuation spectroscopy (FFS), because the wide separation in color between mCherry and green fluorescent protein provides excellent conditions for identifying protein interactions inside cells.

The official biological process of mCherry is bioluminescence and this process allows mCherry to aid in the generation of precursor metabolites and gene expression analytics. mCherry is most commonly used as a fluorescent reporter, acting as a labeled tag …

2023年8月12日 · Unlike mCherry and mRFP, our tests show that the fluorescence lifetimes of eGFP and mCitrine may be influenced not predominantly by crowding, but also by an increase in the local refractive...

Explore Over 2.7k Plasmids: Fluorescent Protein Genes & Plasmids | More Plasmid Sets. BsrGI is typically used at 37°C, but is even more active at 60°C. Efficient cleavage requires at least two copies of the NmeAIII recognition sequence. Sticky ends from different NmeAIII sites may not be …

2006年5月26日 · Spectroscopic and atomic resolution crystallographic analyses of three representatives, mOrange, mStrawberry, and mCherry, reveal that different mechanisms operate to establish the excitation and emission maxima.